Western Blot Evaluation Of LNCaP Cells in Response to Selective Phytochemical Treatments

Abstract:

Phytochemicals present in various plants and plant products instigate definite outcomes on cellular gene targets in living organisms. Many phytochemicals have been recognised for their potentials in chemoprevention and chemotherapy. This study is tasked to establish the effect of curcumin, DIM and EGCG on LNCaP cells to ascertain the effect on selected genes that have been previously reported to be implicated in prostate carcinogenesis. With the application of cell culture treatments and western blotting techniques, the androgen receptor (AR), Akt, Bax, BCL-2, β-catenin, p21, p53, phospho-Akt and surviving proteins were interrogated. Most interestingly is that the androgen receptor protein is observed to be uniquely down-regulated with the DIM treatment and paired combination treatments containing DIM. Selectively, DIM among the other investigated phytochemicals may possess the highest potential in the inhibition of the androgen receptor, a major key player in prostate carcinogenesis.