Acute Ethanol Intoxication in Rats Exposure-Time Dependent, Diphenyl Diselenide Offers a Remedy.

Abstract:

Acute ethanol intoxication causes a lot of pathologies that have been linked to oxidative stress, despite the attending toxicity of ethanol, its acute exposure is sometimes necessitated. Although, a lot of research has investigated acute ethanol intoxication with possible management regimens, especially the use of compounds with antioxidant property, however, less information is available on the time-dependent effects of ethanol on the biochemical indicators of oxidative stress and the possible interactive effects that diphenyl diselenide (DPDSe) may have on it in liver tissues. Hence, this study sought to investigate the time-dependent effects of ethanol and the possible influence DPDSe may have on intoxication at different times.10mg/kg DPDSe was administered orally to white Albino rats 30 minutes before and after oral administration of 13ml/kg of 28% ethanol solution, the reaction was terminated at 1hr, 3hrs, 6hrs, and 12hrs after administration of ethanol for different groups. Thereafter, the oxidative stress indicators such as lipid peroxidation, total thiol, and non-protein thiol as well as activities of enzymes that are stress-responsive such as ⸹-aminolevulinic acid dehydratase (ALAD), 5’-nucleotidase and NTPDase were evaluated. The results of lipid peroxidation, and thiol level show that ethanol-induced stress is more pronounced at times 3hrs and 6hrs of exposure while the protective effect of diphenyl diselenide on the tissues is maximum at 6 hrs after exposure to ethanol. The time-dependence intoxication effect of ethanol on the liver and the time-dependent ameliorative effect of DPDSe show that in the search for more therapeutic agents against acute intoxication of ethanol, time of exposure to ethanol is a valid factor that is to be considered.