GC-MS, Chemical Characterization and Anti-Microbial Activity of Moringa Oleifera Leaf Extract.

Abstract:

The analysis of the leaf of Moringa oleifera was carried out using Gas Chromatography Mass Spectroscopy (GC-MS). Result obtained showed the presence of alkaloids, flavonoids, steroids, tannins, terpenoids and cardiac glycoside. The GC-MS analysis showed that the leaf extract contained 16 compounds which included; Dodecanoic acid (C12H24O2), Tetradecanoic acid, (C14H2802), Hexadecanoic acid, (C17H34O2), n-Hexadecanoic acid, (C16H3202), 9-Octadecanoic acid (C19H36O2), cis-Vaccinic acid, (C18H34O2), Oleic acid, (C18H34O2), Eicosanoic acid, (C21H42O2), Oleic acid, 3-hydroxylpropyl ester (C21H40O3), Oleic acid, (C18H34O2), Docosanoic acid, Methyl ester, (C23H46O2), Vitamin E (C29H50O2), Trioxa-5-phosphaheptacos-18-en-1-aminium,4-hydroxyl-N,N,N-trimethyl-10-oxo-{(1-oxo-9-ocl)}, (C44H84NO8P), Oleic acid, 3-(octadecyloxy) propyl ester, (C39H76O3), Oleic acid, 3-(octadecyloxy) propyl ester, (C39H76O3), 9-Octadecanoicacid [Z]., 2-hydroxyl-1,3-propanedyl ester, (C39H72O5). Similarly, the antimicrobial result showed that the extract had activities against six selected human pathogens such as; Staphylococcus aureus with diameter of inhibition 3.8 and minimum inhibition concentration (MIC) of 50 mg/g, Streptococcus spp with diameter of inhibition 5 and (MIC) 25mg/g, Escherichia coli with diameter of inhibition 20 and (MIC) of 25mg/g, Salmonela. typhi with diameter of inhibition 3 and (MIC) 50, P. aureginosa with diameter of inhibition 3 and (MIC) 50.